| Acetyl-HMGB1

High mobility group box 1 protein, also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a protein that is encoded by the HMGB1 gene.HMG-1 belongs to the high mobility group and contains a HMG-box domain.Like the histones, HMGB1 is among the most important chromatin proteins. In the nucleus HMGB1 interacts with nucleosomes, transcription factors, and histones.[5] This nuclear protein organizes the DNA and regulates transcription.[6] After binding, HMGB1 bends[7] DNA, which facilitates the binding of other proteins. HMGB1 supports transcription of many genes in interactions with many transcription factors. It also interacts with nucleosomes to loosen packed DNA and remodel the chromatin. Contact with core histones changes the structure of nucleosomes.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	5000	2500	1250	625	312.5	156.25	78.12	0
OD	2.15	1.67	1.21	0.85	0.52	0.34	0.21	0.12
Corrected OD	2.03	1.55	1.09	0.73	0.4	0.22	0.09	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human Acetyl-HMGB1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	80-102	97
Plasma(n=8)	92-105	103

 

| Linearity

Samples were spiked with high concentrations of Human Acetyl-HMGB1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-96	87-96
	Average (%)	91	93
1 : 4	Range (%)	89-104	87-108
	Average (%)	94	98